An acellular cerebellar biological scaffold: Preparation, characterization, biocompatibility and effects on neural stem cells.

•A new protocol for effective removal of cellular components from the cerebellum.•The cerebellar scaffold retained neurosupportive proteins and growth factors.•The cerebellar scaffold could stimulate the proliferation and migration of NSCs.•The cerebellar scaffold facilitated the neural differentiation of NSCs.•The cerebellar scaffold showed subcutaneous and intracranial biocompatibility.