Quantification of serum apolipoproteins A-I and B-100 in clinical samples using an automated SISCAPA-MALDI-TOF-MS workflow.

Irene van den Broek,Jan Nouta,Morteza Razavi,Richard Yip,Marco R Bladergroen,Fred P H T M Romijn,Nico P M Smit,Oliver Drews,Rainer Paape,Detlev Suckau,André M Deelder,Yuri E M van der Burgt,Terry W Pearson,N Leigh Anderson,Christa M Cobbaert

Methods（2015）

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摘要

•A fully-automated SISCAPA–MALDI-TOF-MS assay was developed for apoA-I and apoB-100.•The assay allowed processing and analysis of 96 samples in one day.•The imprecision (4 replicates, 5days, 2 sera) was below 5% for both proteins.•Quantification of 93 patient sera agreed well with clinical immunoturbidimetry.•Analytical validation demonstrated selectivity and ruggedness of the assay.

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关键词

Clinical proteomics,Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF),Stable-isotope standards and capture by anti-peptide antibodies (SISCAPA),Cardiovascular disease risk assessment,Analytical method validation

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