A rapid and accurate closed-tube Methylation-Sensitive High Resolution Melting Analysis assay for the semi-quantitative determination of SOX17 promoter methylation in clinical samples.

•SOX17 plays a tumor suppressor role through suppression of Wnt/β-catenin pathway.•SOX17 promoter methylation activates the Wnt signaling pathway.•SOX17 promoter is frequently methylated in many types of cancer.•SOX17 methylation is evaluated so far qualitatively.•We present a semi-quantitative MS-HRMA assay for SOX17 promoter methylation.•SOX17 promoter methylation levels differ between breast cancer samples.