Transfection of live, tick derived sporozoites of the protozoan Apicomplexan parasite Theileria parva.

Ine De Goeyse,Famke Jansen,Maxime Madder,Kyoko Hayashida,Dirk Berkvens,Dirk Dobbelaere,Dirk Geysen

Veterinary Parasitology（2015）

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摘要

•We present the first transient transfection of the parasite.•Plasmid DNA was integrated by nucleofection into fresh purified.•Transfection will facilitate research of the unique host–parasite interactions.

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关键词

Theileria parva,Transfection,Nucleofection,Sporozoites

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