Ornithinibacillus composti sp. nov., isolated from sludge compost and emended description of the genus Ornithinibacillus

A Gram-stain positive, aerobic, motile, endospore-forming and rod-shaped bacterium, designated GSS05 T , was isolated from a sludge compost sample and was characterized by means of a polyphasic taxonomic approach. Growth was observed to occur with 0–3 % (w/v) NaCl (optimum 1 %), at pH 5.5–10 (optimum pH 7.5) and at 15–50 °C (optimum 37 °C). According to the results of a phylogenetic analysis, strain GSS05 T was found to belong to the genus Ornithinibacillus and to be related most closely to the type strains of Ornithinibacillus halotolerans and Ornithinibacillus contaminans (96.5 and 95.1 % 16S rRNA gene sequence similarity, respectively). The peptidoglycan amino acid type was determined to be A4 β . The major respiratory quinone was identified as menaquinone-7 (MK-7). The polar lipid profile of strain GSS05 T was found to contain a predominance of diphosphatidylglycerol, moderate amounts of phosphatidylglycerol and minor amounts of two unknown phospholipids and two unknown lipids. The G+C content of genomic DNA was determined to be 42.1 mol%. The dominant cellular fatty acids were identified as iso-C 15:0 and anteiso-C 15:0 . The phenotypic, chemotaxonomic, phylogenetic and genotypic data indicated that strain GSS05 T represents a novel species of the genus Ornithinibacillus , for which the name Ornithinibacillus composti sp. nov. is proposed. The type strain is GSS05 T (=CCTCC AB 2013261 T = KCTC 33192 T ).