Domain I and II from newly emerging goose tembusu virus envelope protein functions as a dominant-negative inhibitor of virus infectivity.

•The newly emerging duck/goose tembusu virus resulted in serious economic loss in China.•GTV E protein DI/II is sufficient to inhibit JS804 infection in a dose-dependent manner.•DI/II binds to cell membrane and this binding blocks goose tembusu virus E protein access to cell membrane.