Role of Mitofusin-2 in mitochondrial localization and calcium uptake in skeletal muscle.

•Mfn2 knockdown in muscle causes mitochondrial fragmentation and mis-localization.•Mfn2 knockdown in muscle reduces mitochondrial Ca2+ uptake.•Mfn2 knockdown in muscle depolarizes the mitochondrial membrane potential.•Mitochondria in muscle respond to changes in the bulk myoplasmic Ca2+ pool.•Mfn2 is required for optimal mitochondrial Ca2+ uptake in skeletal muscle.