Expression of E2 gene of bovine viral diarrhea virus in Pichia pastoris: a candidate antigen for indirect Dot ELISA.

Yuelan Zhao,Tianyi Ma,Xingyu Ju,Yue Zhang,Min Wang,Teng Liu,Wenbo Cao,Yongzhan Bao,Jianhua Qin

Journal of Virological Methods（2015）

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摘要

•The E2 gene of the BVDV was cloned into the pPIC9K.•The proteins E2 could induce rabbits to produce specific antibodies.•The indirect Dot-ELISA can be used for the detection of antibody against BVDV.

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关键词

Bovine viral diarrhea virus,E2 gene,Immunogenicity,Indirect Dot-ELISA

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