Time-shared experiments for efficient assignment of triple-selectively labeled proteins.

Frank Löhr,Aisha Laguerre,Christoph Bock,Sina Reckel,Peter J Connolly,Norzehan Abdul-Manan,Franz Tumulka,Rupert Abele,Jonathan M Moore,Volker Dötsch

Journal of Magnetic Resonance（2014）

引用 12|浏览8

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摘要

•New experiments improve residue-type distinction in selectively labeled proteins.•Combined acquisition of 2D HN(CX) triple-resonance spectra saves measurement time.•Amide signal overlap is reduced owing to 13C/12C editing into different subspectra.

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关键词

BEST-TROSY,Cell-free expression,Deuteration,Hadamard decoding,Isotope labeling,Membrane proteins,Triple-resonance NMR

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