DNA-PKcs-SIN1 complexation mediates low-dose X-ray irradiation (LDI)-induced Akt activation and osteoblast differentiation.

•LDI increases ALP activity, promotes type I collagen (Col I)/Runx2 mRNA expression.•LDI induces DNA–PKcs activation, which is required for osteoblast differentiation.•Akt activation mediates LDI-induced ALP activity and Col I/Runx2 mRNA increase.•DNA–PKcs–SIN1 complexation mediates LDI-induced Akt Ser-473 phosphorylation.•DNA–PKcs–SIN1 complexation is important for osteoblast differentiation.