CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos

Yanjiao Shao,Yuting Guan,Liren Wang,Zhongwei Qiu,Meizhen Liu,Yuting Chen,Lijuan Wu,Yongmei Li,Xueyun Ma,Mingyao Liu,Dali Li

NATURE PROTOCOLS（2014）

引用 166|浏览15

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摘要

Genetic techniques in rats are developing apace. Here, Shao et al . describe how to use CRISPR/Cas-mediated genome editing to modify one-cell rat embryos and generate stable rat lines.

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关键词

Genetic engineering, Cas9 endonuclease, Rat

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