Precise manipulation of bacterial chromosomes by conjugative assembly genome engineering

Natalie J Ma,Daniel W Moonan,Farren J Isaacs

NATURE PROTOCOLS（2014）

引用 36|浏览7

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摘要

The Isaacs laboratory provides its protocol for CAGE; this method harnesses bacterial conjugation to precisely engineer E. coli genomes to create chimeric strains. A single round of CAGE can be completed in 1 week.

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关键词

Bacterial techniques and applications, Synthetic biology, Genomic engineering

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