Structural analysis of N- and O-glycans using ZIC-HILIC/dialysis coupled to NMR detection.

Yi Qu,Ju Feng,Shuang Deng,Li Cao,Qibin Zhang,Rui Zhao,Zhaorui Zhang,Yuxuan Jiang,Erika M Zink,Scott E Baker,Mary S Lipton,Ljiljana Paša-Tolić,Jian Zhi Hu,Si Wu

Fungal Genetics and Biology（2014）

引用 6|浏览21

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摘要

•We effectively enriched glycans with ZIC-HILIC/dialysis prior to NMR analysis.•We used RNase B for method validation and to aid data interpretation.•We elucidated the N-/O-linked glycan structures of the A. niger secretome using the developed approach.

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关键词

ZIC-HILIC,Dialysis,Glycan,NMR,Secretome,A. niger

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