Enantioselective HPLC determination and pharmacokinetic study of secnidazole enantiomers in rats.

•The first chiral HPLC method for detecting secnidazole in biological samples.•Baseline resolution was achieved on a Chiral-AGP column within 7.0min.•The method was validated and applied to a pharmacokinetic study in rats.•The disposition of secnidazole is not significantly stereoselective in rats.•Chiral inversion and enantiomer–enantiomer interaction do not occur in rats.