Layer-by-layer assembly of chitosan stabilized multilayered liposomes for paclitaxel delivery.

•The multilayered liposomes were synthesized by layer-by-layer assembly.•The addition of chitosan had a stabilization effect on the storage of liposomes.•Chitosan stabilized multilayered liposomes exhibited sustainable release behaviors.•Chitosan stabilized multilayered liposomes revealed enhanced antitumor activity.