Detecting and preventing reversion to toxicity for a formaldehyde-treated C. difficile toxin B mutant.

Bei Wang,Su Wang,Richard R Rustandi,Feng Wang,Christopher D Mensch,Laura Hong,Adam Kristopeit,Susan Secore,Geethanjali Dornadula,Anthony Kanavage,Jon H Heinrichs,Henryk Mach,Jeffrey T Blue,David S Thiriot

Vaccine（2015）

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摘要

•A C. difficile toxin B mutant with five mutations (mTcdB) has residual toxicity.•Formaldehyde can remove residual toxicity but reversion in liquid can occur.•In-vivo mouse toxicity and biochemical assays are sensitive indicators of reversion.•Lyophilization after formaldehyde removal prevents mTcdB reversion to toxicity.•Including formaldehyde can progressively modify mTcdB in the lyophilized state.

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关键词

Toxoid vaccines,Clostridium difficile toxin B

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