Snapback primer mediated clamping PCR for detection of EGFR and KRAS mutations in NSCLC patients by high resolution melting analysis

    BioMed research international, Volume 2014, 2014, Pages 407537-407537.

    Cited by: 5|Bibtex|Views1|Links
    WOS
    Keywords:
    mutationpolymerase chain reaction

    Abstract:

    Assays for detecting somatic mutations are requested with higher sensitivity and more convenience. Here, we describe snapback primer mediated allele clamping enrichment polymerase chain reaction (SPACE-PCR), a novel form of PCR that amplifies minority alleles selectively from mixtures. We replaced regular PCR with SPACE-PCR before sequenc...More

    Code:

    Data:

    Your rating :
    0

     

    Tags
    Comments