Reply To Schmitt Et Al.: Data-Filtering Schemes For Avoiding Double-Counting In Circle Sequencing

Schmitt et al. (1) raise the concern that circle sequencing (2) may spuriously count information from the same starting molecule multiple times. There are indeed several mechanisms by which multiple final reads produced by the circle-sequencing process could be derived from the same starting molecule, as discussed briefly in the last paragraph of our Results section (2). As Schmitt et al. (1) point out, the extent to which this occurs …