Linker mutations reveal the complexity of synaptotagmin 1 action during synaptic transmission

Rapid vesicle exocytosis requires the Ca 2+ sensor synaptotagmin I (syt). Here the AUs manipulate the length and rigidity of the linker connecting the two Ca 2+ sensing domains of syt. This revealed a set of intra-molecular interactions that determined the ability of the domains to penetrate membranes and drive evoked transmitter release in response to Ca 2+ .