Eukaryotic expression systems for structural studies.

Christopher A Nelson,William H McCoy,Daved H Fremont

STRUCTURAL GENOMICS AND DRUG DISCOVERY: METHODS AND PROTOCOLS（2014）

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摘要

This protocol describes protein production in mammalian cells by transient transfection. It assumes the expression construct contains either a 6-HIS or Fc fusion tag to allow recovery of the protein by affinity chromatography. The method is one of the simplest available for protein expression in eukaryotic cells, requires little specialized equipment, and has a reasonably high rate of success.

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关键词

Kozak,Signal sequence,CMV expression vector,HEK293-F cells,Polyethylenimine,PEI,Kifunensine,Transient transfection,Mammalian cell

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