Component co-expression and purification of recombinant human pyruvate dehydrogenase complex from baculovirus infected SF9 cells.

Yong Jiang,Juan Wang,Guofeng Zhang,Khyati Oza,Linda Myers,Marc A Holbert,Sharon Sweitzer

Protein Expression and Purification（2014）

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摘要

•Human PDC complex was successfully expressed in baculovirus infected SF9 cells.•Human PDC was purified in two-steps and the complex was lipoylated in vitro.•This recombinant human PDC showed higher activity in a PDHK enzyme coupled assay.

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关键词

Component co-expression,Pyruvate dehydrogenase,Pyruvate dehydrogenase kinase

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