Transcriptional regulation and functional characterization of the oxysterol/EBI2 system in primary human macrophages.

•Oxysterol metabolizing enzymes are dynamically regulated in macrophages upon LPS challenge.•LPS-induced up-regulation of CH25H and CYP7B1 leads to enhanced release of oxysterols.•LPS induces a transient increase of EBI2 expression in primary human macrophages.•Oxysterols can activate EBI2 endogenously expressed on macrophages and direct their migration.