Winogradskyella Wandonensis Sp Nova, Isolated From A Tidal Flat

A Gram-stain-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated WD-2-2(T), was isolated from a tidal flat of Wando, an island of South Korea, and subjected to a polyphasic taxonomic analysis. Strain WD-2-2(T) grew optimally at 30 degrees C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WD-2-2T belonged to the genus Winogradskyella, clustering coherently with the type strain of Winogradskyella litorisediminis. Strain WD-2-2T exhibited 16S rRNA gene sequence similarity of 97.4% to W. litorisediminis DPS-8(T) and 94.5-96.6% to the type strains of the other species of the genus Winogradskyella. Strain WD-2-2T contained MK-6 as the predominant menaquinone and iso-C-15:1 G, iso-C-17:0 3-OH and iso-C-15:0 as the major fatty acids. The major polar lipids detected in strain WD-2-2(T) were phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid. The DNA GA+C content was 36.4 mol%, and DNA DNA relatedness with W. litorisediminis DPS-8(T) was 13 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain WD-2-2(T) is separate from recognized species of the genus Winogradskyella. On the basis of the data presented, strain WD-2-2T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyefla wandonensis sp. nov. is proposed. The type strain is WD-2-2(T) (=KCTC 32579(T)= CECT 8445(T))