Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages.

•Embryonic stem cells were differentiated into early mesoderm & endoderm lineages.•Major glycosaminoglycanomic changes on these transitions were observed.•Pluripotent H9 cell lumican without glycosaminoglycan chains•Pluripotent H9 cell HS is largely unsulfated while differentiated cell HS is rich in N-sulfo groups.•Dramatic differences were observed in the transcript expression of HS3ST1.