Sequence selective recognition of double-stranded RNA using triple helix-forming peptide nucleic acids.
PEPTIDE NUCLEIC ACIDS: METHODS AND PROTOCOLS, 2ND EDITION(2014)
摘要
Noncoding RNAs are attractive targets for molecular recognition because of the central role they play in gene expression. Since most noncoding RNAs are in a double-helical conformation, recognition of such structures is a formidable problem. Herein, we describe a method for sequence-selective recognition of biologically relevant double-helical RNA (illustrated on ribosomal A-site RNA) using peptide nucleic acids (PNA) that form a triple helix in the major grove of RNA under physiologically relevant conditions. Protocols for PNA preparation and binding studies using isothermal titration calorimetry are described in detail.
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关键词
Double-stranded RNA,Peptide nucleic acids (PNA),Triple helix,Isothermal titration calorimetry (ITC)
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