Negative pseudo-affinity chromatography for plasmid DNA purification using berenil as ligand.

C Caramelo-Nunes,M F Gabriel,P Almeida,J C Marcos,C T Tomaz

Journal of Chromatography B（2014）

引用 6|浏览6

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摘要

•Negative pseudo-affinity chromatography with berenil as ligand.•One-step chromatographic purification of pVAX1-LacZ from clarified E. coli lysates.•Immediate elution of pDNA with a small salt concentration.•Faster DNA plasmid purification method.•Therapeutic grade purification of pDNA.

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关键词

Berenil-Sepharose,Negative pseudo-affinity chromatography,Plasmid purification,Small DNA ligands

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