Ion-pair ultra-high performance liquid chromatographic analysis of monoamines: peak-splitting at high flow rates.

Journal of Chromatography A(2013)

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摘要
•Ion-pair UHPLC at high flow rates results in peak-splitting.•The peak-splitting phenomenon is due to disturbed ion-pair retention mechanisms.•The influence of several chromatographic parameters is investigated.•Under specific conditions, peak-splitting is delayed to higher flow rates.
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关键词
Ion-pair chromatography,UHPLC,Flow rate,Peak-splitting,Monoamines,Electrochemical detection
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