Expression of chicken CTCF gene in COS-1 cells and partial purification of CTCF protein
Biochemistry. Biokhimii͡a(2013)
摘要
The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies.
更多查看译文
关键词
transcription factor CTCF,expression in mammalian cells,COS-1
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要