Development Of An Experimental Model For Assessing The Effects Of Cigarette Smoke And Virus Infections On Inflammatory Responses To Bacterial Antigens
INNATE IMMUNITY(2014)
摘要
Interactions among major risk factors associated with bacterial infections were assessed in a model system using surrogates for virus infection; IFN-g, and exposure to cigarette smoke; cigarette smoke extract (CSE), nicotine and cotinine. Cytokine responses elicited by LPS from THP-1 cells in the presence of these components, or combinations of components, were assessed by multiplex bead assay, i.e. IL-1, IL-6, IL-8, IL-10, TNF- and IFN-. IFN--priming significantly increased pro-inflammatory cytokines induced by LPS. CSE suppressed production of pro-inflammatory cytokines IL-1, TNF- and IFN-, but enhanced production of IL-8. Nicotine and cotinine suppressed all cytokine responses. In combination, IFN- masked the inhibitory effects of CSE. In relation to the objectives of the study, we concluded that (a) IFN- at biologically relevant concentrations significantly enhanced pro-inflammatory responses; (b) CSE, nicotine and cotinine dysregulated the inflammatory response and that the effects of CSE were different from those of the individual components, nicotine and cotinine; (c) when both IFN- and CSE were present, IFN- masked the effect of CSE. There is a need for clinical investigations on the increase in IL-8 responses in relation to exposure to cigarette smoke and increased pro-inflammatory responses in relation to recent viral infection.
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关键词
Lipopolysaccharide, cigarette smoke, virus infection, cytokine responses, THP-1 cells
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