Enzymatic generation of the NO/HNO-releasing IPA/NO anion at controlled rates in physiological media using β-galactosidase.

•We introduce a novel prodrug form of the HNO/NO-releasing IPA/NO anion.•Its hydrolysis by galactosidase is directly proportional to enzyme concentration.•Thus the rate of HNO/NO generation can be precisely programmed.•This enzyme/substrate combination displayed positive inotropism in myocyte culture.