Impact of alg3 gene deletion on growth, development, pigment production, protein secretion, and functions of recombinant Trichoderma reesei cellobiohydrolases in Aspergillus niger.

Ziyu Dai,Uma K Aryal,Anil Shukla,Wei-Jun Qian,Richard D Smith,Jon K Magnuson,William S Adney,Gregg T Beckham,Roman Brunecky,Michael E Himmel,Stephen R Decker,Xiaohui Ju,Xiao Zhang,Scott E Baker

Fungal Genetics and Biology（2013）

引用 25|浏览18

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摘要

•The alg3 gene functions are examined in Aspergillus niger.•Alg3Δ affects growth, development and pigment production.•Alg3Δ enhances the overall protein secretion.•Alg3Δ influences N-linked glycosylation of recombinant rCel7A.•Altered N-glycans of rCel7A improves nanocrystalline cellulose hydrolysis.

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关键词

Asparagine-linked glycosylation 3 (ALG3),Aspergillus niger,Filamentous fungi,N-linked glycosylation,Trichoderma reesei cellobiohydrolase (Cel7A),Protein secretion and expression

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