Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients.

Patricia Costaglioli,Christophe Barthe,Michael Fayon,Naik Christoflour,Stéphanie Bui,Laurence Derlich,Philippe Domblides,Marc Crouzet,Sébastien Vilain,Bertrand Garbay

Molecular and Cellular Probes（2014）

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摘要

The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.

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关键词

Cystic fibrosis,Sputum,Pseudomonas aeruginosa,Reference genes,RT-qPCR

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