[Construction of a plant expression vector with tomato transcription factor gene Pti5 and studies on transgenic tobaccos].

The plasmid pBI121UCH1 carrying Pti5-VP16 gene under the control of the cauliflower mosaic virus 35s promoter was constructed. Leaf segments of tobacco SRI were infected by Agrobacterium tumefaciens EHA105 with plasmid pBI121UCH1, from which kanamycin resistant plants were obtained. PCR and Southern analysis proved that the Pti5-VP16 gene was integrated into the genomes of the tobacco plants. The disease resistance assay showed that the disease resistance was enhanced in the transgenic tobacco plants.