An opaque-2-like transcription factor from pearl millet.

Pearl millet (Pennisetum glaucum L.), a species of the Poaceae family, is all important food crop in Africa, Asia and South America. Its nutritional value is due to storage prolamins accumulated in the seeds. In other species of the same family, the expression of the genes coding for storage prolamins is mediated by the regulatory protein opaque-2. In this paper we show that all opaque-2 -like protein is present in pearl millet too and is expressed during the earl), stages of seed development. The organization of the gene coding for this protein is similar to that of orthologous genes in other Poaceae species, i.e. six exons separated by five introns. A comparison of amino acid homologies with other described opaque-2 proteins is presented.