Chemical Histologic And Immunologic Responses In Rats To Ccl4 By Different Routes Of Administration

SummaryRats given a single, small dose of CCl4 by gastric intubation showed a significant elevation of liver lipids and liver hydroxyproline when sacrificed after 7 days. Rats which received a comparable dose by subcutaneous injection showed only elevated hydroxyproline levels. When the same dose was administered by inhalation neither liver lipids nor hydroxyproline content were increased. The incidence of microscopic lipidoses was greatest in the gastric and subcutaneous groups, while the incidence of necrosis was greater in the gastric and inhalation groups than in the subcutaneous group. A few immune responses were demonstrated by anti-liver hemagglutinations but none by Ouchterlony gel precipitin or skin tests.