rHDL administration increases reverse cholesterol transport in mice, but is not additive on top of ezetimibe or cholestyramine treatment.

•Stimulating cholesterol efflux by rHDL administration rapidly mobilizes cholesterol to plasma.•rHDL significantly increases reverse cholesterol transport (RCT).•Both ezetimibe and cholestyramine significantly enhance RCT.•Inhibiting intestinal sterol uptake is not additive to rHDL administration to increase RCT.