NMR assignment of reduced form of copper, zinc superoxide dismutase from Salmonella enterica

Almost complete assignment (97%) of NMR resonances was obtained for the reduced, Cu(I), form of prokaryotic CuZnSOD from Salmonella enterica . 13 C direct detection was used to complement the standard bouquet of 1 H detected triple resonance experiments and contributed to the identification of proline backbone resonances and to side chains assignments of Asx, Glx and aromatic rings. This is the only complete assignment available for monomer SOD from prokaryotic organisms.