Group VIB Phospholipase A(2) promotes proliferation of INS-1 insulinoma cells and attenuates lipid peroxidation and apoptosis induced by inflammatory cytokines and oxidant agents.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY(2012)
摘要
Group VIB Phospholipase A(2) (iPLA(2)gamma) is distributed in membranous organelles in which beta-oxidation occurs, that is, mitochondria and peroxisomes, and is expressed by insulin-secreting pancreatic islet beta-cells and INS-1 insulinoma cells, which can be injured by inflammatory cytokines, for example, IL-1 beta and IFN-gamma, and by oxidants, for example, streptozotocin (STZ) or t-butyl-hydroperoxide (TBHP), via processes pertinent to mechanisms of beta-cell loss in types 1 and 2 diabetes mellitus. We find that incubating INS-1 cells with IL-1 beta and IFN-gamma, with STZ, or with TBHP causes increased expression of iPLA(2)gamma mRNA and protein. We prepared INS-1 knockdown (KD) cell lines with reduced iPLA(2)gamma expression, and they proliferate more slowly than control INS-1 cells and undergo increased membrane peroxidation in response to cytokines or oxidants. Accumulation of oxidized phospholipid molecular species in STZ-treated INS-1 cells was demonstrated by LC/MS/MS scanning, and the levels in iPLA(2)gamma-KD cells exceeded those in control cells. iPLA(2)gamma-KD INS-1 cells also exhibited higher levels of apoptosis than control cells when incubated with STZ or with IL-1 beta and IFN-gamma. These findings suggest that iPLA(2)gamma promotes beta-cell proliferation and that its expression is increased during inflammation or oxidative stress as a mechanism to mitigate membrane injury that may enhance beta-cell survival.
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关键词
apoptosis,oxidants,cell proliferation,oxidation reduction
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