G-protein coupled receptor 120 is involved in glucose metabolism in fat cells.
CELLULAR AND MOLECULAR BIOLOGY(2012)
摘要
Free fatty acids (FFA) are closely related to insulin resistance in which proteins such as insulin receptor substrate 1 (IRS-1), phosphatidylinositol 3 kinase (PI3K) and glucose transporter type 4 (GLUT4) are involved. Recent researches have shown that G-protein coupled receptor 120 (GPR120) is a receptor for medium and long chain FFA. This study aimed to evaluate the relationship between GPR120 and proteins related to the glucose metabolism. We used siRNA technique to down-regulate the GPR120 expression in 3T3-L1 cells before incubation with palmitic acid (PA), and evaluated the effect of GPR120 expression on the levels of IRS-1, PI3K and GLUT4 after PA induction. RT-PCR and western blot were performed to detect gene and protein levels in differentiated cells. RT-PCR result showed that GPR120 mRNA significantly increased in differentiated cells. GPR120-SiRNA transfection significantly down-regulated GPR120 gene and protein level in differentiated 3T3-L1 cells on day 5 after PA-induced and also decreased lipid droplets accumulated within the cells. SiRNA-mediated decreased of GRP120 was associated with significant reductions in gene and protein levels of IRS-1 and GLUT4. Our results showed that the expression level of GPR120 affected the expression of proteins related to the glucose metabolism, suggesting a contribution of GPR120 in the insulin resistance.
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关键词
Free fatty acids,insulin resistance,G-protein coupled receptor 120,GPR120,glucose metabolism
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