Iterative plug-and-play methodology for constructing and modifying synthetic gene networks

Kevin D Litcofsky, Raffi B Afeyan,Russell J Krom,Ahmad S Khalil,James J Collins

NATURE METHODS（2012）

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摘要

unique multiple cloning site (MCS) and defined genetic components without MCS restriction sites allow for the rapid construction and iterative tuning of synthetic genetic circuits.

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关键词

Synthetic Biology, Molecular Biology, Molecular Engineering, Systems Biology

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