E- And Z-Isomers Of New Phytoecdysteroid Conjugates From French Polynesian Microsorum Membranifolium (Polypodiaceae) Fronds

Phytochemical investigation of the fronds of Microsorum membranifolium resulted in the isolation of a new phytoecdysteroid, E-2-deoxy-20-hydroxyecdysone 3-[4-(1-beta-D-glucopyranosyl)]-caffeate (1), together with two known phytoecdysteroids, E-2-deoxy-20-hydroxyecdysone 3-[4-(1-beta-D-glucopyranosyl)]-ferulate (2), E-2-deoxyecdysone 3-[4-(1-beta-D-glucopyranosyl)]-ferulate (3). Their respective Z-isomers 4-6 were also observed and identified for the first time. The new structures were elucidated on the basis of extensive spectroscopic data analysis (1D, 2D-NMR and HR-MS techniques).