Thermal Tolerance And Physiological Changes In Mud Crab, Scylla Paramamosain Crablet At Different Water Temperatures

Simple SummaryThe nursery phase of mud crab (Scylla spp.)-from megalopa to several, further crablet stagesneeds to be carried out in optimal environmental conditions, until they reach a larger size suitable to being stocked to a pond. This study observed the behavioral thermoregulation of crablet instar phases, and determined the effects of several levels of water temperature on growth, survival, molting cycle, gill condition, and sex ratio in the nursery phase of S. paramamosain. In this study, optimal temperature for the long-term nursery phase of S. paramamosain, beginning with megalopa stage, was found to be 28 to 30 degrees C, with the possibility that water temperature could affect the sex ratio of mud crabs. Findings of this study could result in increased crablet production from hatcheries, and assist in realizing potential of monosex seed production of S. paramamosain, through temperature treatment.This study was carried out to determine the physiological changes (survival, growth, molting cycle, sex differentiation, and gill condition) of mud crab, Scylla paramamosain crablet at different water temperatures of 24, 28 and 32 degrees C, and ambient temperature of 27 to 30 degrees C. Thermoregulatory behavior, represented by preferred temperature (29.83 +/- SD 2.47 degrees C), critical thermal minimum (17.33 +/- SD 0.58 degrees C), critical thermal maximum (40 +/- SD 0.00 degrees C), and thermal tolerance interval (22.67 +/- SD 0.58 degrees C), were checked for Crablet 1 stage only (with ambient temperature as acclimation temperature).Both low (24 degrees C) and high (32 degrees C) temperatures were associated with lower growth performance, and survival rate (p < 0.05), in comparison with both 28 degrees C and ambient temperature treatments.Male ratio at low temperaturetreatment (24 degrees C) was higher (80.09 +/- SD 18.86%) than for other treatments (p < 0.05), observed as 44.81 +/- D 10.50%, 41.94 +/- SD 19.44%, and 76.30 +/- SD 5.13% for 28 degrees C, 32 degrees C and ambient temperature treatments, respectively. However, there was no significant difference observed between 24 degrees C, 28 degrees C, and ambient temperature treatments. Anatomical alterations of gill lamellae of S. paramamosain crablet for both 32 degrees C, and 24 degrees C treatments, appeared thinner and paler than at both 28 degrees C, and ambient temperature treatments. Based on this study, temperature of 28 to 30 degrees C was recommended as the optimal temperature for the long-term nursery phase of S. paramamosain.