Two virus strains of isolated in China are recombinant alphaviruses]

To determine the classification, phylogenetic and genetic type of XJ-90260 and XJ-91006 viruses isolated in China.Two pairs of specific primers were designed to amplify the NSP4 and E1-3' UTR genes of XJ-90260 and XJ-91006 viruses and the nucleotide sequences between them and with that of other Alphavirus were compared.The nucleotide sequences identity between XJ-90260 and XJ-91006 viruses was 100% and has showed the highest with WEE, comparing with the other 26 Alphaviruses in 3'-UTR. NSP4 gene of XJ-90260 and XJ-91006 viruses high homologous with EEE virus and E-1 gene high homologous wih SIN virus. XJ-90260 and XJ-91006 viruses belong to the B group of WEE and are closest to the Russian strain (Y62-33) by phylogenetic analysis.XJ-90260 and XJ-91006 viruses isolated in China are recombinant Alphaviruses, they belong to the same genetic type as WEE does.