Optimization of a whole blood intracellular cytokine assay for measuring innate cell responses to mycobacteria.
Journal of Immunological Methods(2012)
摘要
Innate cells are essential for host defense against invading pathogens, and the induction and direction of adaptive immune responses to infection. We developed and optimized a flow cytometric assay that allows measurement of intracellular cytokine expression by monocytes, dendritic cells (DC) and granulocytes, as well as cellular uptake of green-fluorescent protein (GFP)-expressing mycobacteria, in very small volumes of peripheral blood.
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关键词
Mycobacteria,Flow cytometry,Monocytes,Dendritic cells,Granulocytes,Innate cytokines
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