Expression and Purification of Recombinant Proteins Using the Baculovirus System.

Cheryl Isaac Murphy,Helen Piwnica-Worms, Stefan Grünwald,William G Romanow,Nicole Francis,Hua-Ying Fan, Sharon Marr

Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]（2018）

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摘要

This article describes how to analyze protein expression in cells infected with recombinant baculovirus on a small scale for optimizing protein production, how to maximize and scale up recombinant protein production, and how to purify recombinant proteins. © 2018 by John Wiley & Sons, Inc.

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baculovirus,expression,purification,recombinant protein

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