Development of a TaqMan assay for the six major genotypes of hepatitis C virus: comparison with commercial assays

    Journal of medical virology, Volume 80, Issue 1, 2008, Pages 72-79.

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    Keywords:
    viral loadtaqmangenotypes

    Abstract:

    A quantitative real-time PCR assay was developed that detects genomic RNA from reference strains representing the six major genotypes of hepatitis C virus (HCV) with equal sensitivity and accurately measured HCV RNA in JFH1 HCV-infected Huh7.5 cells. The method is indirectly calibrated to the first international (WHO 96/790) HCV standard ...More

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