Molecular analysis of the Bacteroides fragilis recA gene

The nucleotide sequence of a 2.5-kb DNA segment containing the Bacteroides fragilis recA gene was determined. The coding region of the recA gene specifies a protein of 318 amino acids. The RecA protein of B. fragilis shows significant homology with that of Escherichia coli, Thiobacillus ferooxidans, Pseudomonas aeruginosa and Proteus mirabilis. No SOS box characteristic of LexA-regulated promoters could be identified in the 5′-noncoding region of the B. fragilis recA gene. Promoter activity of the cloned recA gene in E. coli was located within a 113-bp fragment of the B. fragilis DNA by in vitro construction of operon fusions with a promoterless lacZ gene. The transcription start point for this gene in B. fragilis was determined by primer extension analysis.