Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis.

Andreas Matussek,Lisa Stark,Olaf Dienus,Joakim Aronsson,Sara Mernelius,Sture Löfgren,Per-Eric Lindgren

JOURNAL OF CLINICAL MICROBIOLOGY（2011）

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摘要

We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.

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关键词

staphylococcus aureus,electrophoresis,clinical diagnostics

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