RP-HPLC analysis of Jirakadyarishta and chemical changes during fermentation.

Jirakadyarishta an Ayurvedic formulation prepared by the fermentation of a decoction of Cuminum cyminum (seeds) is traditionally used for intestinal disorders RP-HPLC analysis of the decoction and the final processed formulation revealed that apigenin-7-O-[galacturonide (1 -> 4)-O-glucoside] and luteolin-4 -O-glucoside-7-O-galacturonide) were the two major constituents of the decoction of C cyminum Selective hydrolysis of 7-O-glucosides of luteolin and apigenin during fermentation resulted in an increase in the amount of luteolin and apigenin The 4 -O-glucoside-7-O-galacturonide of luteolin and galacturonide derivative of apigenin were not hydrolyzed during fermentation Monomeric phenolics, together with 5-hydroxymethyl furfural (5-HMF), were also introduced into the formulation through the jaggery and other plant materials during fermentation This communication highlights the importance of the ancient processing methods used in Ayurveda