An Alternative Method To Determining Optical Lever Sensitivity In Atomic Force Microscopy Without Tip-Sample Contact

Force studies using atomic force microscopy generally require knowledge of the cantilever spring constants and the optical lever sensitivity. The traditional method of evaluating the optical lever sensitivity by pressing the tip against a hard surface can damage the tip, especially sharp ones. Here a method is shown to calculate the sensitivity without having to bring the tip into contact. Instead a sharpened tungsten wire is used to cause a point contact directly onto the cantilever and cause cantilever bending. Using beam theory, the sensitivity thus found can be converted to the equivalent sensitivity that would be obtained using the tip location. A comparison is presented between sensitivity values obtained from the conventional tip contact method and those derived from the wire-based technique for a range of cantilevers in air. It was found that the difference between the calculated sensitivity from the wire-based technique and the sensitivity obtained conventionally was less than 12%. These measurements indicate the presented method offers a simple alternative approach to obtain optical lever sensitivity without compromising the tip shape. (C) 2010 American Institute of Physics. [doi:10.1063/1.3459886]